Frequency of Acyclovir-Resistant Herpes Simplex Virus in Clinical Specimens and Laboratory Isolates
AUTOR(ES)
Shin, Young Kyoo
FONTE
American Society for Microbiology
RESUMO
The proportion of acyclovir (ACV)-resistant herpes simplex virus (HSV) isolates in clinical specimens and laboratory isolates was determined. HSV isolates in clinical specimens and laboratory isolates were cultured in the absence or presence of 5 μg of ACV per ml. The frequency of ACV-resistant HSV was calculated as (average virus titer in the wells with ACV)/(average virus titer in the wells without ACV). The mutation frequency of HSV type 1 isolates in clinical samples (directly from patient lesions) was 7.5 × 10−4 ± 2.5 × 10−4 (mean ± standard error), and that of HSV type 2 isolates was 15.0 × 10−4 ± 4.6 × 10−4. The mutation frequencies of isolates derived in the laboratory from these clinical samples were very similar. Similarly, the 50% inhibitory concentrations for isolates in clinical samples and laboratory isolates were identical. The frequencies of ACV-resistant HSV types 1 and 2 were in a narrow range of 7.5 × 10−4 to 15.0 × 10−4 among isolates in clinical specimens and did not change for laboratory-derived pools of viral isolates.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=87849Documentos Relacionados
- In vitro activity of penciclovir against clinical isolates of acyclovir-resistant and foscarnet-resistant herpes simplex virus.
- High-Frequency Phenotypic Reversion and Pathogenicity of an Acyclovir-Resistant Herpes Simplex Virus Mutant
- Improved DNA hybridization method for detection of acyclovir-resistant herpes simplex virus.
- Acyclovir diphosphate dimyristoylglycerol: a phospholipid prodrug with activity against acyclovir-resistant herpes simplex virus.
- Drug combinations for treatment of mice infected with acyclovir-resistant herpes simplex virus.
