Freezing Injury and Phospholipid Degradation in Vivo in Woody Plant Cells: I. Subcellular Localization of Phospholipase D in Living Bark Tissues of the Black Locust Tree (Robinia Pseudoacacia L.) 1
AUTOR(ES)
Yoshida, Shizuo
RESUMO
The subcellular localization of phospholipase D in homogenates of living bark tissues of the black locust tree (Robinia pseudoacacia L.) was examined and found in both soluble and particulate fractions. At least some of the soluble enzyme was considered to be compartmentalized in vacuoles. Considerable amounts of phospholipase D seemed to be tightly bound on several membranes such as endoplasmic reticulum, tonoplast, and a membrane associated with potassium-stimulated ATPase (pH 6.1). The mitochondrial fraction banding at the 40 to 43% (w/w) sucrose layer, however, had the lowest specific activity. The soluble and the particulate phospholipase D were considered to be similar in nature. It is possible that the particulate enzyme, as a part, may be derived from the coexisting nonvesiculated materials visualized in the electron micrograph of each membrane fraction. An involvement of the soluble or the presumed membrane-bound phospholipase D in phospholipid degradation in vivo during freezing at sublethal temperatures was discussed with special reference to freezing injury of plant cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=543063Documentos Relacionados
- Freezing Injury and Phospholipid Degradation in Vivo in Woody Plant Cells: III. Effects of Freezing on Activity of Membrane-bound Phospholipase D in Microsome-enriched Membranes 1
- Freezing Injury and Phospholipid Degradation in Vivo in Woody Plant Cells: II. Regulatory Effects of Divalent Cations on Activity of Membrane-bound Phospholipase D 1
- Purification and Characterization of an α-Glucosidase from Rhizobium sp. (Robinia pseudoacacia L.) Strain USDA 4280
- Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage
- Studies on the Chemistry of the Living Bark of the Black Locust Tree in Relation to Frost. VI. Amylase and Phosphorylase Systems of the Bark Tissues1