Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.

AUTOR(ES)

Weber, H

RESUMO

We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the alpha 2 interferon gene on the one side and a portion of the alpha 1 interferon gene (homology about 80%) on the other, were transfected into E. coli SK1592. Appropriate resistance markers allowed the isolation of colonies containing circular plasmids which arose by in vivo recombination between the partly homologous interferon gene sequences. Eleven different recombinant genes were identified, six of which encoded new hybrid interferons not easily accessible by recombinant DNA techniques.

Documentos Relacionados

• The inhibition by chloramphenicol of nascent protein formation in E. coli.
• The characterization of the EBV alkaline deoxyribonuclease cloned and expressed in E. coli.
• The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.
• Stabilization of proteins by a bacteriophage T4 gene cloned in Escherichia coli.
• Priming immunization against cholera toxin and E. coli heat-labile toxin by a cholera toxin short peptide-beta-galactosidase hybrid synthesized in E. coli.