Formamide Sensitivity: A Novel Conditional Phenotype in Yeast
AUTOR(ES)
Aguilera, A.
RESUMO
Yeast mutants unable to grow in the presence of 3% formamide have been isolated in parallel with mutants sensitive to either 37° or 6% ethanol. The number of formamide-sensitive mutations that affect different genes that can be identified from yeast cells is at least as large as the number of thermosensitive or ethanol-sensitive mutations. These mutations are of two types: those that are sensitive to formamide, temperature and/or ethanol simultaneously; and those that are specific for formamide sensitivity and show no temperature or ethanol sensitivity phenotype. Those genes susceptible to giving rise to formamide-sensitive alleles include the structural gene for DNA ligase, CDC9, and the structural gene for arginine permease, CAN1. The results indicate that formamide sensitivity can be used as a novel conditional phenotype for mutations on both essential and nonessential genes. This work also confirms that ethanol-sensitivity can be used as a conditional phenotype to identify mutations in at least as many genes as those susceptible to temperature or formamide sensitive mutations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1205795Documentos Relacionados
- Symptom Sensitivity: Its Social and Cultural Correlates
- Organic solvent exposure and contrast sensitivity: comparing men and women
- Occupational asthma from nickel sensitivity: I. Human serum albumin in the antigenic determinant.
- Severe intrauterine growth retardation with increased mitomycin C sensitivity: a further chromosome breakage syndrome.
- Conditional Mutants of Meiosis in Yeast