Follow up of a robust meta-signature to identify Zika virus infection in Aedes aegypti: another brick in the wall
AUTOR(ES)
Fukutani, Eduardo, Rodrigues, Moreno, Kasprzykowski, José Irahe, Araujo, Cintia Figueiredo de, Paschoal, Alexandre Rossi, Ramos, Pablo Ivan Pereira, Fukutani, Kiyoshi Ferreira, Queiroz, Artur Trancoso Lopo de
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
28/05/2018
RESUMO
The mosquito Aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. In a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, West Nile and yellow fever viruses. Of these 110 genes, four genes allowed a highly accurate classification of infected status. More recently, a new study of Ae. aegypti infected with Zika virus (ZIKV) was published, providing new data to investigate whether this “infection” gene set is also altered during a ZIKV infection. Our hypothesis is that the infection-associated signature may also serve as a proxy to classify the ZIKV infection in the vector. Raw data associated with the NCBI/BioProject were downloaded and re-analysed. A total of 18 paired-end replicates corresponding to three ZIKV-infected samples and three controls were included in this study. The nMDS technique with a logistic regression was used to obtain the probabilities of belonging to a given class. Thus, to compare both gene sets, we used the area under the curve and performed a comparison using the bootstrap method. Our meta-signature was able to separate the infected mosquitoes from the controls with good predictive power to classify the Zika-infected mosquitoes.
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