Fluorescência óptica no diagnóstico de lesões teciduais / Optical fluorescence in tissue injury diagnosis

AUTOR(ES)

Vitória Helena Maciel Coelho

DATA DE PUBLICAÇÃO

2005

RESUMO

The fluorescence spectroscopy is a conventionally used method, in the study of materials for the compound determination in function to the characteristic emission bands. The same principle can be used to determine the presence of fluorescent agents in biological tissues, which can be useful in the diagnosis, and for a better direction in the treatment of tissue injuries. From the tissue injuries, the injuries by ultraviolet and muscle injuries, they deserve a special attention due to their frequent occurrence. The injuries by ultraviolet can induce skin cancer, which nowadays represents the highest incidence in Brazil and the muscle injuries are the most common, when compared to the other pathologies that affect human beings, that is why these two kinds of injuries will be studied in this paper. Although, thus, the fluorescence spectroscopy has shown to be efficient in the detection of superficial injuries, doubts remain about the technique sensitivity for interstitial injuries (which happen deeply), this study aims to evaluate the efficiency and sensitivity of the technique in the differentiation between normal tissue and injured tissue, for the diagnosis of these injuries. The system used to get the diagnosis uses a laser source of excitation in 442 nm (Helium-Cadmium), a tip of detection with a central fiber and six peripheral ones to collect the re-emitted light from the tissue, a monochromator and a computer. For the research, Wistar rats weighting about 300 g was used, which are injured using an ultraviolet lamp and traumatized, using a direct non-invasive trauma equipment. The results was be obtained through comparative analysis between the spectrums from normal tissue and injured ones.

ASSUNTO(S)

injury by ultraviolet and muscle injury fluorescence diagnostic diagnóstico fluorescência spectroscopy lesão por ultravioleta e lesão muscular espectroscopia

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