Fluorescence detection of specific sequence of nucleic acids by oxazole yellow-linked oligonucleotides. Homogeneous quantitative monitoring of in vitro transcription.
AUTOR(ES)
Ishiguro, T
RESUMO
We have developed a fluorescent DNA probe, oxazole yellow (YO)-linked oligonucleotide complementary to a target DNA/RNA, which can enhance the fluorescence on hybridizing with a target nucleotide. We demonstrated the applicability of the YO-linked oligonucleotide probe to real-time monitoring of the in vitro transcription process of a plasmid DNA constructed containing the 5'-terminus non-coded region of hepatitis C virus RNA. In the process of in vitro transcription in the presence of YO-linked complementary oligonucleotide, the fluorescence of the reaction mixture showed a time-dependent linear increase corresponding to the generated target RNA product.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146341Documentos Relacionados
- Rapid detection of sequence variations using polymers of specific oligonucleotides.
- Sequence-targeted chemical modifications of nucleic acids by complementary oligonucleotides covalently linked to porphyrins.
- Specific inhibition of transcription by triple helix-forming oligonucleotides.
- Hybridization-based affinity partitioning of nucleic acids using PEG-coupled oligonucleotides.
- Transcription-mediated binding of peptide nucleic acid (PNA) to double-stranded DNA: sequence-specific suicide transcription.