First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association

AUTOR(ES)

Gusmão, Desiely Silva, Santos, Adão Valmir, Marini, Danyelle Cristine, Russo, Érica de Souza, Peixoto, Anelise Maria Dias, Bacci Júnior, Maurício, Berbert-Molina, Marília Amorim, Lemos, Francisco José Alves

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2007-12

RESUMO

We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.

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