First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes
AUTOR(ES)
Figueiredo-Carvalho, Maria Helena Galdino, Ramos, Livia de Souza, Barbedo, Leonardo Silva, Chaves, Alessandra Leal da Silva, Muramoto, Ilda Akemi, Santos, André Luis Souza dos, Almeida-Paes, Rodrigo, Zancopé-Oliveira, Rosely Maria
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2016-01
RESUMO
This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management.
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