Fingerprint and multi-ingredient quantitative analyses for quality evaluation of hawthorn leaves and Guang hawthorn leaves by UPLC–MS

AUTOR(ES)

Xu, Baoxin, Yang, Mingyu, Du, Yilong, Zhao, Shengnan, Li, Yanrong, Pan, Haifeng

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

2018-06

RESUMO

ABSTRACT This paper aimed to evaluate the quality of hawthorn leaves and Guang hawthorn leaves by an UPLC–MS method from two aspects, fingerprint analysis and multi-ingredient quantification. Chromatographic separation was carried out on an UPLC system, the standardized characteristic fingerprints was established by Similarity Evaluation System for chromatographic fingerprinting of traditional Chinese medicine and cluster analysis. Eight components were simultaneously determined by mass spectrometry in multiple reaction-monitoring mode. The method was validated in terms of linearity (R2 > 0.9971), intraday and interday precision (RSD < 2.0%), repeatability (RSD < 2.3%), stability (RSD < 2.5%) and recovery (96.2–103.8%). The developed method was successfully applied to the quality evaluation between hawthorn leaves and Guang hawthorn leaves, and there were differences in the component and the content, hawthorn leaves and Guang hawthorn leaves cannot substitute each other in clinical medication.

Documentos Relacionados

• UPLC–QTOF–MS and NMR analyses of graviola (Annona muricata) leaves
• Pesticide Residues Method Validation by UPLC-MS/MS for Accreditation Purposes
• Validation and application of an analytical method for the determination of mycotoxins in crackers by UPLC-MS/MS
• UPLC/Q-TOF-MS profiling of phenolics from Canarium pimela leaves and its vasorelaxant and antioxidant activities
• Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration