Ferredoxin-1 mRNA is destabilized by changes in photosynthetic electron transport

AUTOR(ES)

Petracek, Marie E.

FONTE

The National Academy of Sciences

RESUMO

In transgenic tobacco, pea Ferredoxin-1 (Fed-1) mRNA accumulates rapidly in response to photosynthesis even when the transgene is driven by a constitutive promoter. To investigate the role of photosynthesis on Fed-1 mRNA stability, we used the tetracycline repressible Top10 promoter system to specifically shut off transcription of the Fed-1 transgene. The Fed-1 mRNA has a half-life of approximately 2.4 hr in the light and a half-life of only 1.2 hr in the dark or in the presence of the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). These data indicate that cessation of photosynthesis, either by darkness or DCMU results in a destabilization of the Fed-1 mRNA. Furthermore, the Fed-1 mRNA half-life is reduced immediately upon transfer to darkness, suggesting that Fed-1 mRNA destabilization is a primary response to photosynthesis rather than a secondary response to long-term dark adaptation. Finally, the two different methods for efficient tetracycline delivery reported here generally should be useful for half-life measurements of other mRNAs in whole plants.

Documentos Relacionados

• The 5′ End of the Pea Ferredoxin-1 mRNA Mediates Rapid and Reversible Light-Directed Changes in Translation in Tobacco1
• The IS10 transposase mRNA is destabilized during antisense RNA control.
• Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis.
• Facilitation of dendritic mRNA transport by CPEB
• Photoreduction of ferredoxin with various electron donors: Support for the Z scheme of photosynthetic electron transport