Fate of DNA in eclipse complex during genetic transformation in Streptococcus pneumoniae.
AUTOR(ES)
Vijayakumar, M N
RESUMO
Uptake of DNA and genetic recombination proceeded normally in competent Streptococcus pneumoniae despite inhibition of DNA replication by 6-(p-hydroxyphenylazo)-uracil. Immediately after a brief uptake period, 68% of donor DNA label was in eclipse complex form, and 22% was in low-molecular-weight products; by the completion of integration at 10 min, 23% was integrated into the chromosome, and the rest was lost from the cell. Throughout the process, less than 1% was found as free single strands. The DNA in eclipse complex is therefore an intermediate in the integration process.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217878Documentos Relacionados
- Use of a cloned DNA fragment to analyze the fate of donor DNA in transformation of Streptococcus pneumoniae.
- Transformation of encapsulated Streptococcus pneumoniae.
- An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
- LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae.
- Transformation of restriction endonuclease phenotype in Streptococcus pneumoniae.
