Extração liquido-liquido de xilanase por micela reversa numa microcoluna de campanulas pulsadas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

In this work, it was studied a microcolumn agitated by puIsed caps, due to promote an efficient contact between the phases in the column and also to avoid the enzyme denaturation and the loss of main proteins properties. This work deals with the purification of xylanase produced by Penicillium janthinellum by liquid-liquid extraction on reverse micelles, to micellar inner, utilizing a cationic surfactant BDBAC (N-benzyI-N-dodecyl-N-bis(2-hydroxyethyl)). It was used design statistical with the intention of realized selection of the main variables in the process: frequency pulsed, volumetric flow and ionic strength; the response surface methodology was employed to the quantified levels of this. This resulted in two major mathematical models: one representing the use of pure enzyme and the other the use of enzymatic extract. It was predict and observed experimentally for both models, obtained activity yield in the order of 140% to the pure enzyme and 43% to the enzymatic extract. It was observed in this work that, the methodology statistical employed was extremely important and that the microcolumn used were stable operation and that high activity yield was reached

ASSUNTO(S)

enzimas proteinas - separação extractors (chemical engineering) extração - equipamento proteins enzymes experimental design planejamento experimental

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