Expression of thin aggregative fimbriae promotes interaction of Salmonella typhimurium SR-11 with mouse small intestinal epithelial cells.
AUTOR(ES)
Sukupolvi, S
RESUMO
The factors that mediate binding of Salmonella typhimurium to small intestinal epithelial cells have not been fully characterized. In this paper we demonstrate that elimination of production of thin aggregative fiber by a transposon insertion within the gene encoding the subunit protein of the fiber reduced binding of S. typhimurium SR-11 to a conditionally immortalized proximal small intestinal epithelial cell line established from transgenic mice. This binding defect could be overcome by transcomplementation with a wild-type allele. The conditionally immortalized cell line should prove useful in identifying the epithelial cell receptor for bacterial attachment since expression of its bacterial binding activity can be induced by manipulating the line's proliferative status.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=175765Documentos Relacionados
- Extracellular Polysaccharides Associated with Thin Aggregative Fimbriae of Salmonella enterica Serovar Enteritidis
- Salmonella enteritidis agfBAC operon encoding thin, aggregative fimbriae.
- Purification and characterization of thin, aggregative fimbriae from Salmonella enteritidis.
- Thin, aggregative fimbriae mediate binding of Salmonella enteritidis to fibronectin.
- A PhoP-repressed gene promotes Salmonella typhimurium invasion of epithelial cells.