Expression of tandem gene fusions in transgenic tobacco plants.
AUTOR(ES)
Dean, C
RESUMO
We have studied the expression of four sets of tandem gene fusions in transgenic tobacco plants. This was to determine if the problem of between-transformant variability in expression of introduced genes could be overcome by using a linked reference gene as a co-ordinately expressed control. Tandem gene fusions containing identical 5' flanking regions (SSU301-ocs with either SSU301-cat or SSU301-SSU911) were not co-ordinately expressed in the transgenic tobacco plants whereas the tandem gene fusions containing similar but not identical 5' flanking regions (SSU301-ocs with SSU911-cat or SSU911-SSU301) were co-ordinately expressed. The lack of co-ordinate expression of some of the tandem gene fusions appears to be partially explained by absence of the corresponding genomic DNA segments in the transgenic plants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338429Documentos Relacionados
- Gene fusions to lacZ reveal new expression patterns of chimeric genes in transgenic plants.
- Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants.
- Peroxidase-Induced Wilting in Transgenic Tobacco Plants.
- Maize sucrose synthase-1 promoter directs phloem cell-specific expression of Gus gene in transgenic tobacco plants.
- Expression of functional replication protein from tomato golden mosaic virus in transgenic tobacco plants.