Expression of bacterial virulence factors and cytokines during in vitro macrophage infection by enteroinvasive Escherichia coli and Shigella flexneri: a comparative study
AUTOR(ES)
Bando, Silvia Y, Moreno, Ana CR, Albuquerque, José AT, Amhaz, Juliana MK, Moreira-Filho, Carlos A, Martinez, Marina B
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2010-09
RESUMO
Enteroinvasive Escherichia coli (EIEC) and Shigellaspp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigellashare many genetic and biochemical similarities, the illness caused by Shigellais more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneriwith cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed thatS. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri.
Documentos Relacionados
- Factors Affecting Virulence of Shigella flexneri: Defective Methionine Synthesis in an Escherichia coli-Shigella Hybrid
- Factors Affecting Virulence of Shigella flexneri: Strain with Relaxed Control of Ribonucleic Acid Synthesis
- Factors Affecting Virulence of Shigella flexneri: Avirulent Strain with Altered Metabolism of Succinate, Fumarate, and Malate
- Virulence plasmids of enteroinvasive Escherichia coli and Shigella flexneri integrate into a specific site on the host chromosome: integration greatly reduces expression of plasmid-carried virulence genes.
- A 101-kilodalton heme-binding protein associated with congo red binding and virulence of Shigella flexneri and enteroinvasive Escherichia coli strains.