Expression and immune recognition of the conserved MSP4 outer membrane protein of Anaplasma marginale.
AUTOR(ES)
Oberle, S M
RESUMO
Defining conserved, protective epitopes is essential to the design of an effective vaccine against bovine anaplasmosis. MSP4, one of six initial body proteins recognized by a neutralizing serum, is conserved among Anaplasma marginale isolates at both the protein and the DNA levels. Sera from cattle immunized with an outer membrane fraction of A. marginale and protected from a virulent challenge bind MSP4. The gene for MSP4 has been cloned, and the recombinant protein has been expressed, isolated, and demonstrated to share epitopes with the native protein expressed on initial bodies. MSP4 may have a greater potential to protect cattle from a challenge by heterologous isolates than other A. marginale surface proteins, which vary widely in size and structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=281308Documentos Relacionados
- Phylogeography of msp4 genotypes of Anaplasma marginale in beef cattle from the Brazilian Pantanal
- Analysis of membrane protein genes in a Brazilian isolate of Anaplasma marginale.
- Simultaneous Variation of the Immunodominant Outer Membrane Proteins, MSP2 and MSP3, during Anaplasma marginale Persistence In Vivo
- The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species.
- CD4+ T Lymphocytes from Anaplasma marginale Major Surface Protein 2 (MSP2) Vaccinees Recognize Naturally Processed Epitopes Conserved in MSP3