Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
AUTOR(ES)
Taha, Siti Aisyah Mohd, Ling, Joanne Koh Su, Azmi, Nur Izyan Binti, Kannan, Thirumulu Ponnuraj, Azlina, Ahmad, Mokhtar, Khairani Idah
FONTE
Braz. J. Oral Sci.
DATA DE PUBLICAÇÃO
2015-06
RESUMO
To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.
Documentos Relacionados
- Notch signaling is a direct determinant of keratinocyte growth arrest and entry into differentiation
- Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients
- Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway
- Activation of Notch signaling pathway precedes heart regeneration in zebrafish
- Tissue-specific gene expression in mouse hepatocytes cultured in growth-restricting medium.
