Expressao da ciclooxigenase-2 (COX-2) e do fator de necrose tumoral (TNF-) na viabilidade de retalho cutaneo randomico em ratos apos a irradiacao laser de baixa intensidade / Expressao da ciclooxigenase-2 (COX-2) e do fator de necrose tumoral (TNF-) na viabilidade de retalho cutaneo randomico em ratos apos a irradiacao laser de baixa intensidade / Expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-alpha (TNF-á) on viability of random skin flaps in rats after low level laser therapy / Expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-alpha (TNF-á) on viability of random skin flaps in rats after low level laser therapy

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

26/01/2011

RESUMO

Objective: To investigate the effect of low intensity laser irradiation on viability of random skin flap in rats and to evaluate the concentration levels of COX-2 and TNF- á. Method: The sample consisted of 24 rats Wistar EPM-1 randomly divided into three groups. All animals were subjected to a surgically induced cranial base dorsal skin flap, measuring 10X4 cm. In group A, it was performed a simulation of laser irradiation; group B was subjected to laser irradiation at 20mW and 0.29J; group C was subjected to laser irradiation at 100mW and 7.30J. These procedures were performed in the cranial half of the flap, and repeated in all three groups at different postoperative periods (POI, PO2 and PO1). The percentage of necrosis was measured on the seventh postoperative day by the paper template method. To evaluate the immunohistochemical expression of COX-2 and TNF-á, three pieces of tissue were collected in the proximal, middle and distal parts of the flap. The immunohistochemical evaluation was performed using the streptavidin-biotin method, and the expression of COX-2 and TNF-á was quantified in scores from 0 to 3, with (0) negative, (1) weak, (2) moderate and (3) strong. Results: The area of necrosis was lower in group C compared to groups A and B (p<0.05) and no difference was found between groups A and B. The concentration levels of COX-2 and TNF-á were lower in the proximal point, in group C compared to groups A and B (p<0.05). Conclusion: The low level laser was effective in increasing the viability of random skin flap in rats when a protocol of 7.30J of energy was applied, probably due to the inflammatory response regulation demonstrated by the lower expression of COX-2 and TNF-á observed at the base of the flaps.

ASSUNTO(S)

terapia laser de baixa intensidade retalhos cirúrgicos fator de necrose tumoral alfa ciclooxigenase 2 ratos cirurgia experimental

ACESSO AO ARTIGO

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