Expressão da ciclo-oxigenase-2 e do Ki67 em lesões precursoras do cancer do colo uterino

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Objectives: To assess the expression of cyclooxygenase-2 (COX-2) in squamous lesions of the uterine cervix and its possible relations to nuclear expression of Ki67, Human papillomavirus (HPV) detection and to the persistence/recurrence of cervical intraepithelial neoplasia (CIN) after diathermic conization. Subjects and methods: This was a prospective study with intermediate cross-sectional analysis, in which women subjected to diathermic conization due to cervical abnormalities were selected between February 2001 and April 2004. The first article is a cross sectional study, including surgical specimens of 164 women subjected to conization. Cytoplasmic expression of COX-2 and nuclear expression of Ki67, ascertained through immunohistochemistry, was evaluated according to cervicallesion grade and high-risk HPV detection through Hybrid Capture 11. In the second article, 104 women ith CIN were included, followed-up for 36 months after diathermic conization, in order to assess possible relation between COX-2 and Ki67 expression, adjusted by the cone margin status and high-risk HPV detection during follow-up with persistentlrecurrent CIN. Results: Cross-sectional analysis of baseline data disclosed that COX-2 protein expression did not differ in relation to grade of cervical lesion, Ki67 expression and high-risk HPV detection. A higher expression of Ki67 was associated exclusively with CIN 3. In the follow-up series, fourteen cases of recurrentlpersistent CIN were detected after diatherrny conization. Persistence/recurrence of CIN were not related to cytoplasmic COX-2 and nuclear Ki67 expression, but had a positive association with high-risk HPV detection during follow-up (Risk Ratio 7.6; 95% confidence interval 2.1-28.6). Conclusion: The cytoplasmic expression of COX 2 did not correlate with cervical lesion grade, nuclear Ki67 expression and high-risk HPV detection. COX-2 and Ki67 expression was not related to persistence/recurrence of CIN

ASSUNTO(S)

neoplasia intra-epitelial cervical imunohistoquimica colo uterino

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