Experimental infection with Neospora caninum in young dogs (Canis familiaris), adults and in pregnant bitches. / Infecção experimental por Neospora caninum em cães (Canis familiaris) jovens, adultos e em cadelas gestantes.

AUTOR(ES)

Guacyara Tenorio Cavalcante

DATA DE PUBLICAÇÃO

2010

RESUMO

The objectives of this study were to evaluate transplacental transmission of N. caninum in different stages of pregnancy (Exp I) and evaluate different bovine tis.sues as means of transmission of N. caninum in young dogs and adults (Exp II). In Exp I, Three dogs were inoculated with 108 tachyzoites of N. caninum in the third week of gestation, three at 6 sixth week and one remained as a control. All infected dogs, and at least one of their offspring, seroconverted to anti-N. caninum antibodies by Immunofluorescence Assay. There was presence of the parasite by Hematoxylin- Eosine exam in Central Nervous System and by PCR and RFLP ITS-1 in lymph node, brain, heart and liver. In Exp II, young and adult dogs received different tissues of cattle naturally infected with N. caninum: heart, brain, liver and masseter. None seroconverted. Only the young dogs shed oocysts of N. caninum by eating masseter (2 dogs, 40%), heart (2 dogs, 40%), liver (one dog, 33%) and brain (3 dogs, 75%).

ASSUNTO(S)

bitches cães dogs oocistos pregnancy infecções por protozoários protozoan infections oocysts cadelas gravidez neospora caninum neospora caninum

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