Evidence that there are only two tRNA(Phe) genes in Escherichia coli.
AUTOR(ES)
Pittard, J
RESUMO
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate mutase P-prephenate dehydratase, the first enzyme in the terminal pathway of phenylalanine biosynthesis, was derepressed. Similar derepression of pheA has been reported in pheR mutants of E. coli K-12 (J. Gowrishankar and J. Pittard, J. Bacteriol. 150:1130-1137, 1982). Attempts to introduce a pheR mutation into the delta pheV strain failed under circumstances suggesting that this combination of mutations is lethal. Southern blot analysis of pheV+ and delta pheV strains indicated that there are only two tRNA(Phe) genes in E. coli. It is recommended that the names pheU and pheV be retained for these genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=526932Documentos Relacionados
- Suppression of a double missense mutation by a mutant tRNA(Phe) in Escherichia coli.
- Selection for active E. coli tRNA(Phe) variants from a randomized library using two proteins.
- Expression of a set of synthetic suppressor tRNA(Phe) genes in Saccharomyces cerevisiae.
- Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA.
- Sequence of the mitochondrial control region, tRNA(Thr), tRNA(Pro) and tRNA(Phe) genes from the black rhinoceros, Diceros bicornis.
