Evidence that Auxin-induced Growth of Soybean Hypocotyls Involves Proton Excretion 1
AUTOR(ES)
Rayle, David L.
RESUMO
The role of H+ excretion in auxin-induced growth of soybean hypocotyl tissues has been investigated, using tissues whose cuticle was rendered permeable to protons or buffers by scarification (scrubbing). Indoleacetic acid induces both elongation and H+ excretion after a lag of 10 to 12 minutes. Cycloheximide inhibits growth and causes the tissues to remove protons from the medium. Neutral buffers (pH 7.0) inhibit auxin-induced growth of scrubbed but not intact sections; the inhibition increases as the buffer strength is increased. Both live and frozen-thawed sections, in the absence of auxin, extend in response to exogenously supplied protons. Fusicoccin induces both elongation and H+ excretion at rates greater than does auxin. These results indicate that H+ excretion is involved in the initiation of auxin-induced elongation in soybean hypocotyl tissue.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=440648Documentos Relacionados
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