Evidence for sequence-specific conformational changes in DNA from the melting temperatures of DNA phosphorothioate derivatives.
AUTOR(ES)
Suggs, J W
RESUMO
Analogs of alternating purine-pyrimidine DNA polymers such as poly(dA-dT)-poly(dA-dT) can be made with phosphorothioate groups in the DNA backbone. A phosphorothioate diester at the 5'-purine-pyrimidine-3' step causes a significant lowering of the polymer's melting temperature compared to a phosphorothioate diester at the 5'-pyrimidine-purine-3' step. This may occur because sulfur substitution increases anionic charge density in the DNA minor groove and 5'-purine-pyrimidine-3' steps narrow the minor groove. The ability to modulate charge density in the DNA backbone via sulfur substitution should prove useful in studies of sequence-dependent conformational changes in DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321900Documentos Relacionados
- Sequence-specific binding of echinomycin to DNA: evidence for conformational changes affecting flanking sequences.
- Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA
- Sequence-specific DNA modification in Neisseria gonorrhoeae.
- Sequence-specific DNA uptake in Haemophilus transformation.
- Peptide antisera as sequence-specific probes of protein conformational transitions: calmodulin exhibits calcium-dependent changes in antigenicity.
