Evidence for 30-40S RNA as Precursor of the 60-70S RNA of Rous Sarcoma Virus

AUTOR(ES)

Canaani, E.

RESUMO

Rous sarcoma virus harvested from cells at intervals of 3 min has the same density, sedimentation coefficient, and DNA polymerase as virus harvested at hourly intervals. The RNA of the Prague strain-C consists of a minor class of 60-70S RNA, a major class 30-40S RNA, and a 4-12S class of RNA present at variable concentration. The RNA of the Schmidt-Ruppin strain-A contains more 60-70S than 30-40S RNA. Upon incubation of virus harvested at 3-min intervals at 40° in cell growth medium or Tris-saline, most of the 30-40S RNA is converted to 60-70S RNA.

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