Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.
AUTOR(ES)
Swierkosz, E M
RESUMO
An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 isolates were correctly typed by direct immunofluorescence assay (Kallestad Laboratories, Inc.), but 1 of 39 HSV-2 isolates did not react with the HSV-2 type-specific antibody conjugate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=271739Documentos Relacionados
- Evaluation of three immunofluorescence assays for culture confirmation and typing of herpes simplex virus.
- Comparison of immunofluorescence with commercial monoclonal antibodies to biochemical and biological techniques for typing clinical herpes simplex virus isolates.
- Clinical evaluation of commercial conjugates for direct immunofluorescence of herpes simplex virus.
- Comparison of an avidin-biotin immunoassay with three commercially available immunofluorescence kits for typing of herpes simplex virus.
- Identification and typing of herpes simplex virus by enzyme immunoassay with monoclonal antibodies.