Evaluation of the rapid decarboxylase and dihydrolase test for the differentiation of nonfermentative bacteria.

AUTOR(ES)

Oberhofer, T R

RESUMO

A rapid medium for the detection of lysine and ornithine decarboxylase and arginine dihydrolase activity of 439 strains of gram-negative, nonfermenting bacteria was evaluated and compared with Moeller decarboxylase medium. Results were obtained in 4 to 24 h using the rapid medium, whereas Moeller medium often required extended (3 to 7 days) incubation. There was 100% agreement in the lysine tests with both media and almost 100% agreement in the ornithine tests. There was 91% agreement in the arginine tests, with the significance of discrepant results discussed. The sensitivity, specificity, and quick results obtained by the rapid test make it a suitable substitute for Moeller medium for the identication of gram-negative, nonfermenting bacteria.

Documentos Relacionados

• Use of the API rapid NFT system for identifying nonfermentative and fermentative marine bacteria.
• Fluorogenic substrates for differentiation of gram-negative nonfermentative and oxidase-positive fermentative bacteria.
• A rapid method for determining decarboxylase and dihydrolase activity
• Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria.
• Comparison of spot esculin hydrolysis with the PathoTec strip test for rapid differentiation of anaerobic bacteria.