Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus)

AUTOR(ES)

Pinto, Tatiana Maria Farias

FONTE

Anim. Reprod.

DATA DE PUBLICAÇÃO

25/11/2019

RESUMO

Abstract The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed’s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.

Documentos Relacionados

• The early development of the goat (Capra hircus)
• Avaliação do Teste Cervical Comparativo no diagnóstico imunoalérgico da tuberculose em caprinos (Capra hircus)
• Teste de tuberculinização em caprinos (Capra hircus) experimentalmente sensibilizados
• Avaliação da morfometria renal através do método ultrassonográfico de fêmeas caprinas (Capra hircus) normais da raça Saanen
• Ocorrência da infecção por Cryptosporidium spp. em cabritos (Capra hircus)