Evaluation of the addition of glycerol to Cupriavidus necator culture medium over Poly(3-hydroxybutyrate) production

AUTOR(ES)

Apati, Giannini Paszinick, Kelbert, Maikon, Sombrio, Bruna Regina, Schneider, Andrea Lima dos Santos, Garcia, Michele Cristina Formolo, Furigo Junior, Agenor, Pezzin, Ana Paula Testa

FONTE

Matéria (Rio J.)

DATA DE PUBLICAÇÃO

05/03/2018

RESUMO

ABSTRACT Glycerol was used as a source of additional carbon in the production of Poly(3-hydroxybutyrate) (P(3HB)). The inverted sugar and glycerol concentrations and the temperature of the Cupriavidus necator culture medium were evaluated using a Central Composite Rotational Design (CCRD). The results showed that the increase in temperature and sugar concentration led to an increase in production and P(3HB) accumulation and when 15 g L−1 of glycerol was added better results were obtained, however these were not considered statistically significant. The best results were obtained at 38 °C and with 30 g L−1 of inverted sugar. Although not considered statistically significant, the addition of 15 g L−1 of glycerol increased the P(3HB) accumulation percentage by 15 %, thus in kinetic terms, greater productivity was obtained in 0.32 g L−1 h−1 polymer. Larger scale assays are being conducted to verify if the addition of glycerol improves the thermal and mechanical properties of the synthesized polymer.

Documentos Relacionados

• Evaluation of the addition of glycerol to Cupriavidus necator culture medium over Poly(3-hydroxybutyrate) production
• POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Cupriavidus necator SUPPLEMENTED WITH MINIEMULSIFIED SOYBEAN OIL
• High cell density strategy for poly(3-hydroxybutyrate) production by Cupriavidus necator
• Production of poly(3-hydroxybutyrate) by fed-batch culture of filamentation-suppressed recombinant Escherichia coli.
• Photodegradation and Photostabilization of Poly(3-Hydroxybutyrate)