Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile

AUTOR(ES)

Spigaglia, Patrizia

FONTE

American Society for Microbiology

RESUMO

Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.

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