Evaluation of Phagocytosis Function of Corynebacterium pseudotuberculosis from Goats naturally infected with Caprine Arthritis-Encephalitis Vírus / Avaliação da função fagocítica de células da linhagem monócitos-macrófagos de caprinos naturalmente infectados pelo vírus da artrite-encefalite caprina, à Corynebacterium pseudotuberculosis

AUTOR(ES)

Barbara Gabriela Soares Sanches

DATA DE PUBLICAÇÃO

2008

RESUMO

To evaluate the phagocytosis from monocyte-macrophage line cells 30 naturally infected Saanen goats with caprine arthritis-encephalitis vírus (VCAE) were used, and divided uniformly in different groups according to agar gel immunodiffusion test (AGID). Peripheral Blood Mononuclear Cell (PBMC) was isolated by density gradient centrifugation. The monocyte-macrophage cells were separated from PBMC by their adherence properties. Afterwards, the phagocytosis function was assessed by phagocytosis assay using Corynebacterium psudotuberculosis as a source of antigen. Therefore, two distinct types of phagocytosis were observed and were set according to the number of bacteria within the cytoplasmatic vacuoles. Thus, the phagocytosis rates were also divided in two groups, on the first was observed up to 12 bacteria in the vacuoles; on the other hand in the second group an uncountable number of bacteria were usually seen. Consequently, the phagocytosis rates were also divided in phagocytosis + (up to 12 bacteria) and phagocytosis ++ (more than 12 bacteria). The results from the phagocytosis rates show any difference, however when the phagocytosis rates were separated in the order of the number of Corynebacterium pseudotuberculosis phagocyted, the phagocytosis ++ from positive animals in the sera test were higher than the negative one (p <0.001). Nevertheless, the negative group presents higher pahgocytosis + (p <0.012). Furthermore a positive and significant correlation between phagocytosis ++ and monocyte phagocytosis (p <0.006) were also encountered in the positive animals, however the same were not observed in the negative group (p <0.49). In face of, the results from the present trial point out to higher susceptibility to caseous linphadenitis in goats infected with VCAE due to the alteration on the phagocytosis strength in these animals.

ASSUNTO(S)

corynebacterium pseudotuberculosis phagocytosis fagocitose corynebacterium pseudotuberculosis caprine arthritis-encephalitis vírus caseous limphadenitis artrite encefalite caprina a vírus linfadenite caseosa macrófagos caprine macrophages caprinos

Documentos Relacionados

• Forma nervosa da artrite-encefalite caprina
• Soroconversão e sororeatividade de cabras leiteiras naturalmente expostas ao virus da artrite-encefalite caprina no Brasil
• Estudo da transmissão do vírus da artrite-encefalite caprina em cabritos neonatos
• Influência da carga viral na transmissão sexual do Vírus da Artrite-Encefalite Caprina.
• PERFIL HEMATOLÓGICO E BIOQUÍMICA SÉRICA DE CABRAS F1 ANGLO-NUBIANA X SAANEN EM LACTAÇÃO SOROPOSITIVAS E SORONEGATIVAS PARA O VÍRUS DA ARTRITE-ENCEFALITE CAPRINA