Evaluation of immunoregulatory and apoptosis and gene polymorphisms in patients with preeclampsia. / Avaliação de polimorfismos de genes codificadores de mediadores de imunorregulação e apoptose em pacientes com pré-eclâmpsia.
AUTOR(ES)
Karen Priscilla Tezotto Pendeloski
DATA DE PUBLICAÇÃO
2010
RESUMO
Purpose: to evaluate the relationship between CTLA-4 (+49), CD28 (+17), ICOS (-1564), Fas (-670) and FasL (-844) gene polymorphisms and susceptibility to preeclampsia (PE) in its mild and severe form. Methods: This case-control study included 130 preeclamptic women and 261 control pregnant women without any obstetric or systemic disorders, with a history of at least two previous successful pregnancies. Genomic DNA was extracted from whole blood using DTAB/CTAB method, and polymorphisms genotyping were obtained by PCR-RFLP technique. Data were analyzed by Students t, qui-square (c2) and Fischers exact tests, and the level of significance was set at p<0.05. Results: Genotype frequencies of ICOS (-1564) polymorphism were 41.9% TT, 48.4% TC and 9.7% CC in the PE group; and 59.3% TT, 32.3% TC and 8.4% CC in controls (p=0.01). Genotype frequencies of Fas (-670) polymorphism were 19.8% AA, 45.2% AG and 34.9% GG in the PE group; and 62% AA, 50.2% AG and 24.5% GG in controls (p=0.09). FasL (-844) genotype frequencies were 23.8% TT, 42.3% TC and 33.8% CC in the PE group; and 33.5% TT, 46.5% TC and 20% CC in controls (p=0.007). There were no significant differences between CTLA-4 and CD28 genotypic frequencies, when comparing women with PE and controls (c2 test: p=0.62, p=0.38, respectively). When comparing mild and severe PE, there were significant differences in CTLA-4 polymorphism (c2 test: p=0.03). There were no significant differences detected between the clinical patterns of PE in CD28, ICOS, Fas and FasL polymorphisms genotypic distribution (c2 test: p=0.33, p=0.28, p=0.5 and p=0.91 respectively). Conclusions: There is evidence of an association between ICOS (-1564), Fas (- 670) and Fas-L (-844) polymorphisms and the occurrence of PE. Our data suggests that CTLA- 4 (+49) polymorphism is related to the occurrence of different clinical patterns of PE, i.e., ifmild or severe forms. We did not identify any association between CD28 (+17) polymorphism and PE.
ASSUNTO(S)
3. tolerância imunológica 1. pré-eclâmpsia ginecologia e obstetricia 2. polimorfismos genéticos 4. apoptose.
