Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry

AUTOR(ES)

Barbosa, Joana, Rodrigues, Acácio Gonçalves, Pérez, Maria José, Pina-Vaz, Cidália

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

21/11/2014

RESUMO

Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.

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