A Comparative Evaluation of the Level of Concanavalin A Binding by Enriched Plasma Membrane Fractions From Developing Soybean Roots
AUTOR(ES)
Berkowitz, Robert L.
RESUMO
The Concanavalin A (Con A) binding capacity of plasma membranes isolated from meristematic and mature regions of four-day-old soybean (Glycine max L. Merr. cv. Wells) roots was compared. Con A binding was studied using a radiochemical assay with tritiated (3H)-Con A and by an electron microscope technique using Con A-ferritin (Con A-F). In both cases, plasma membranes isolated from meristematic tissue bound significantly more Con A than did corresponding membranes from mature tissue. The relative difference in reactivity, as determined by the two procedures, was approximately 49% (3H-Con A) and 46% (Con A-F). In contrast, Km values, determined from 3H-Con A binding curves, were approximately the same, indicating that receptor sites on plasma membranes from both sources were qualitatively similar.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426214Documentos Relacionados
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