Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
AUTOR(ES)
Thomas, Anvy Susan, Saravanakumar, Rupachandra, Gupta, Pratiksha V.
FONTE
Rev. bras. farmacogn.
DATA DE PUBLICAÇÃO
2017-02
RESUMO
ABSTRACT Biologically active proteins isolated from plant species can be used in traditional medicine as prolific resources for new drugs Morinda pubescens Sm., Rubiaceae, is a promising medicinal plant which is widely used in folk medicine to treat fever due to primary complex, ulcer and glandular swellings. In this study, proteins were extracted from the leaves of M. pubescens, and precipitated with ammonium sulphate at various saturation concentrations ranging from 20 to 80%. The precipitated protein sample obtained with 80% saturation was further purified using ultrafiltration membrane (<10 kDa). SDS-PAGE analysis identified the presence of crude and ultrafiltered protein bands. FTIR spectrum of the ultrafiltered protein fractions depicted the presence of hydroxyl and carbonyl groups of proteins. The ultrafiltered proteins exhibited increased cytotoxic activity on A549 cells at the concentrations ranging from 15 to 100 µg/ml. About 98% cell viability was also observed in Vero cells treated with the maximum concentration of 100 µg/ml of ultrafiltered protein extract. DNA fragmentation was observed in A549 cells treated with 10 µg/ml of ultrafiltered proteins, indicating the onset of apoptosis.
Documentos Relacionados
- Cytotoxic activity of phenolic constituents from Echinochloa crus-galli against four human cancer cell lines
- In vitro and in vivo evaluation of NCX 4040 cytotoxic activity in human colon cancer cell lines
- Antiproliferative activity of methanol extracts of four species of Croton on different human cell lines
- Phytochemical study and evaluation of cytotoxic and genotoxic properties of extracts from Clusia latipes leaves
- Volatile compounds of Nectandra salicina (lauraceae) from Costa Rica and their cytotoxic activity on cell lines
