Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

AUTOR(ES)

Bono, James L.

FONTE

American Society for Microbiology

RESUMO

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g−1 in artificially inoculated bovine feces following enrichment.

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