Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

AUTOR(ES)

Éboli, Lígia Patrícia de Carvalho Batista, Netto, Alcides Augusto Salzedas, Azevedo, Ramiro Antero de, Lanzoni, Valéria Pereira, Paula, Tatiana Sugayama de, Goldenberg, Alberto, Gonzalez, Adriano Miziara

FONTE

Acta Cir. Bras.

DATA DE PUBLICAÇÃO

2016-12

RESUMO

ABSTRACT PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) . RESULTS: Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION: King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.

Documentos Relacionados

• Intestinal Glucose Uptake Protects Liver from Lipopolysaccharide and d-Galactosamine, Acetaminophen, and Alpha-Amanitin in Mice
• Glomerular hemodynamics in established glycerol-induced acute renal failure in the rat.
• A bioartificial liver to treat severe acute liver failure.
• Hypophosphataemia in acute liver failure.
• ACUTE LIVER FAILURE