ETIQUETAS DE SECUENCIAS EXPRESADAS DIFERENCIALES DE FRUTOS DE AGUACATE RAZA MEXICANA (Persea americana Mill. var. drymifolia)

AUTOR(ES)

Sánchez-González, Enrique Ignacio, Hernández-Delgado, Sanjuana, Aguirre-Arzola, Víctor Eustorgio, Torres-Castillo, Jorge Ariel, Gutiérrez-Díez, Adriana

FONTE

Rev. Bras. Frutic.

DATA DE PUBLICAÇÃO

15/02/2018

RESUMO

ABSTRACT In this study, Differential Expressed Sequence Tags (ESTs) of immature fruits of Persea americana Mill var. drymifolia of the state of Nuevo León, Mexico were developed and identified. Ten genotypes with fruits of different size and shape were selected to generate ESTs by the differential display technique. In total, 393 amplified differential fragments were obtained, 82 fragments were sequenced and edited for identification and comparison with NCBI nucleotide and protein databases. Forty sequences showed significant similarity to mRNA sequences and / or sequences of hypothetical or predicted proteins belonging to P. americana and / or other genera. Some sequences were related to enzymes such as flavanone-3-hydroxylase (F3H), lecithin-cholesterol acyltransferase, glutathione-S-transferase microsomal and pleiotropic drug resistance protein. With the information of the nucleotide composition of ESTs, primers could be designed to quantify expression levels by real-time RT-PCR of genes in different phenological stages of the fruit and to make comparisons among genotypes that allow determining alternative uses of their fruits.

Documentos Relacionados

• Genitalia de tres especies de Heilipus Germar (Coleoptera: Curculionidae) que dañan frutos de aguacate (Persea americana Mill) en México y Costa Rica
• Estudo experimental da cinética de secagem do abacate (Persea americana Mill.)
• Polifenoloxidase e peroxidase na polpa de abacate (Persea americana Mill.)
• Tolerância a baixas temperaturas de cultivares de abacate (Persea americana Mill.)
• The avocado oil (Persea americana Mill) as raw material for food industry