Estudos sobre sistemas de liberaÃÃo controlada da vacina antiaftosa e do cobalto II para uso veterinÃrio

AUTOR(ES)
DATA DE PUBLICAÇÃO

1996

RESUMO

On the first stage of our work, suspended virus O1 Campos (causing subtype of virus of foot-and-mouth disease) prepared by VALLÃE S/A was proposed as ligands to be bound to the Starch through covalents or by using carbodiimide in order to produce crosslinkings. The starch is oxidated by sodium metaperiodate 0,4 N in pH 4,5, that vicinal hidroxlicos groups are converted into aldehydic, allowing that viral O1 suspensions be immobilized in this support. Diciclohexilcarbodiimida (DCC) in a lightly alkaline pH makes crosslinks among the proteins of these suspended viruses. The Vaccine against foot and mouth disease (FMD) free and immobilized were submitted to the chromatographic and spectrophotometric assays. In these tests 7,5mg of proteins were applied in a column packed with Sephadex G-100 and this allow us to verify the immobilization of the 01 Campos virus through its increase in the absorption bands at 280nm and a volume decrease of the volume elution. The adsorbed vaccine in aluminum hydroxide (control group) and the immobilized one were applied of 5 ml intramuscular injection in cattles aiming to carry through tests of serum protection index (ISP) to assure the liberation of the O1 campos virus into the animal organism. The ISP results had shown that the system Starch + virus suspended is suitable for the controlled liberation of the antigen, mainly in the last sixty days of experiment. On the second stage the complexation of cobalt (II) was proposed with carbohydrates and/or proteins as systems of controlled liberation from this metal for the use of animal feed. Spectrophotometric and chromatographic comparative studies were carried out between a commercial product (Cobalt- Dextrose-Lactose) whose structure was unknown and a product made in our laboratory. For the chromatographic analysis, patterns, samples, solvent systems and appropriate developer with composition were used. The results evidenced the cobalt (II) complexation with glucose. For the spectrophotometric analysis salt solutions of cobalt (II) pure or with glucose and lactose were used with a variation of carbohydrates concentrations and pH of the preparations. Cobalt (II) Chloride with proteins and activated. Furthermore, cassava starch were analyzed with sodium metaperiodate with 0,4 N in several concentrations although the pH of preparations were kept. The results of the spectrophotometric studies showed molar relationships between ligands and cobalt (II) which are necessary for the complexation in the commercial product (Cobalt- Dextrose-Lactose) and the product made in our laboratory. In the commercial product (Cobalt- Dextrose-Lactose) the conditions for the formation of the Cobalt- Dextrose-Lactose complex are unfavorable, due to molar relationship between [carbohydrates] / [cobalt II] be approximately 1,73. Our product, glucose to be used as a ligand for the complexation of the cobalt II, needs a molar relationship [glucose] / [cobalt II] greater than 10. The mixed glucose with protein in a molar relationship of only 2,5 x 10-2 presents a high power of complexation. Protein and activated cassava starch as metaperiodate make the complexation with cobalt II easier even if it owns a molar relationship thousand of times inferior when it is compared to cobalt II complexated with glucose

ASSUNTO(S)

complexaÃÃo cobalto ii vacina antiaftosa bioquimica antiaftosa vaccine cobalt ii complexation

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