Estudos de interação de β2-glicoproteína I em solução aquosa e com interfaces lipídicas / Study of interaction between β2- glycoprotein I in aqueous solution and with lipid interfaces

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

β2GPI is a blood glycoprotein circulating free or bound to lipoproteins. β2GPI adsorbs to negatively charged surfaces, acting as anticoagulant and modulator of inflammation. This work was designed to characterize the interaction between protein molecules and among protein molecules and lipid surfaces. The β2GPI SDS-PAGE and immunoblot revealed monomer, dimer and oligomers. Light scattering methods (static, dynamic and X-ray) showed that β2GPI generates aqueous solutions of anisometric macroaggregates. Atomic force microscopy showed that β2GPI dried on muscovite surfaces assembles itself in prolate ellipsoids. The particle shape and size depended both on the pH and protein concentration. The interaction among β2GPI and lipid surfaces was studied by microgravimetry. β2GPI adsorption to pure phosphatidylcholine was weaker than to gold or phosphatidylcholine/phosphatidylserine surfaces. Artificial lipoproteins adsorbed to β2GPI in a pH dependent manner. Results suggest that β2GPI biological effects could be mediated by protein-protein interactions and lipid surface binding, and pH dependent.

ASSUNTO(S)

macromolecules espalhamento de luz dinâmico glycoprotein macromoléculas glicoproteína. β2-glicoproteína i espalhamento de luz estático bioquímica clínica static light scattering β2-glycoproteini dynamic light scattering

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