Estudos de genes envolvidos na via biossintética do antibiótico antitumoral Cosmomicina / Genes study envolved in biosynthetic pathway of antitumoral antibiotic Cosmomycin.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Cosmomycin is an antitumoral antibiotic produced by the soil bacteria Streptomyces olindensis DAUFPE 5622. Gene expression studies established that stress condition genes like dnaJ and 18hsp, and cosmomycin biosynthetic pathways genes are expressed under antibiotic production. Also the genes cosS and cosY (unknowns function), were selected and analyzed by bioinformatics techniques attributing a transcriptional regulator and ornithine cyclodeaminase functions, respectively. A cassette was constructed in order to inactivate these two selected genes and generating void mutants. Another gene cosK, with glycosiltransferase function, also presented differences in its expression when the antibiotic is produced. We described in this work the presence of a hypothetical glycosiltransferase related with B-daunosamine daunomy, which transfers sugar molecules in the biosynthesis of daunomycin antibiotic.

ASSUNTO(S)

streptomyces olindensis genes reguladores regulator genes glycosyltransferase biblioteca genômica genomic library polyketide antibiotics antibióticos glicosiltransferases policetídeos streptomyces olindensis

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