Estudos comparativos de efeito Bohr em hemoglobinas de Osteoglossum bicirrhossum e Pterygoplicthys pardalis
AUTOR(ES)
Maria Isabel Galdames Portus
DATA DE PUBLICAÇÃO
1977
RESUMO
Two species of fish hemoglobin from Amazon river were ànalized in order to establish the relationship between their molecular and physiological properties as well as their interactions with others molecules and ions. The species were different regarding respiration, metabolic actívity and habitat. The polyacrylámide electrophoresis pattern of Osteoglossum bicirrhossum hemoglobin showed only one component and pronounced Bohr and Root effect and the Rill coefficient showed significant variation with the pH in the presence or not of 10-3M ATP, with the lowestaffinity at pH 7.4. The total hemolisate of Pterygoplicthys pardalis presented no Bohr effect neverthless the four isolated components showed different functional properties thus the HbI component, isolated by DEAE-SEPHADEX showed small reverse Bohr effect that normal in the presence of 10-3M ATP at low pH. The other becomes three omponents presented normal Bohr effect and ATP does not change this effect. From these findings was possible to establish that the Osteoglossum bicirrhossum, a very active fish that lives iu stream with water-breathing type of respiration exihibits only one hemoglobin with high Bohr and Root effect while Pterygoplícthys pardalis a bottom físh both air and water breathing types of respiration have two family of hemoglobins with different functional properties. These results clearly show the capacity of molecular adaptation of fish hemoglobin to adapt to both the metabolic requirements and environment variations of the animals
ASSUNTO(S)
pesquisa biologica hemoglobina - estudos experimentais
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=vtls000055976Documentos Relacionados
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