Estudo microbiológico fecal de linhagens de camundongos, de estirpes de E. coli e do meio ambiente em biotérios / Fecal microbiologic study of mice lineages, E. coli strains and the environment in laboratory animal facilities

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Mice have been largely used at experiences since 17o century, so their microbiological quality should be investigated and kept safety in order to avoid they become ill or die during an investigation and dont transmit zoonosis to those who handle them; this is important in order to make the results presented in the experience be reliable. The Escherichia coli is part of enteric microbiota of mammals, and some of them may cause infections. The pathogenic strains of E. coli show different virulence factors, such as endotoxins, adhesins, enterotoxins, necrotizing citotoxic factors (cnf) and hemolysins. This study has aimed to analyze: the bacterial and fungi aerobic microbiota present at intestine of lineages Swiss, C57BL/6, BALB/c, C3H/HePas, C3H/HeJ, MDX and YCx43 mice, checking if there are differences among them; to evaluate the susceptibility in vitro to antimicrobial agents of E. coli isolated, checking if there are differences among lineages; to verify the occurrence of resistance to multiple antimicrobial agents at E. coli isolated; to investigate the virulence factors of lineages of E. coli, also checking if there are differences among these lineages and the lineages studied; identify the micro-organisms presented at different environments where these animals are kept, checking if there are differences among them. The mice were submitted to necropsy and it was collected a little amount of their fecal content. This fecal content was seeded in culture mediums of BHI, sheep blood agar 5%, MacConkey agar and Saboraud dextrose agar. The mices lineages studied have different fecal microbiotas; the E. coli strains are different in each lineage and theyve showed resistance to multiple antibiotics; the E. coli strains are not pathogenics; isolated bacteria on animal rooms were not affected by mices fecal microbiotas, the microbiological monitoring of animals and biosafety standards always must be adopted and followed at animal rooms.

ASSUNTO(S)

teste de sensibilidade microbiana mice escherichia coli animal strains biotério escherichia coli linhagens animais camundongos laboratory animal facilities microbian sensibility test

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