"Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial" / Laboratory study of the wound healing response to epithelial scrape injury in the human cornea

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Purpose: To examine the early wound healing response to epithelial scrape in human corneas. Methods: Normal corneas had epithelial scrape prior to enucleation. Histology, TUNEL assay, Ki67, SMA and transmission electron microscopy were performed. Results: Epithelial scrape was performed in six corneas from ½ to 65 hours prior to preservation. Keratocyte apoptosis was detected in the anterior stroma in all scraped corneas. Keratocyte proliferation was detected exclusively 65 hours after scrape. No myofibroblast was detected. One cornea was not scraped (control). Conclusion: Results obtained in human corneas (keratocyte apoptosis and proliferation) were similar to animal models

ASSUNTO(S)

apoptosis microscopia de fluorescÊncia/métodos estroma corneal/fisiopatologia epitÉlio da cÓrnea/cirurgia epitÉlio da cÓrnea/crescimento &desenvolvimento estroma corneal/anatomia &histologia epithelium corneal/surgery microscopy confocal/methods estroma corneal/citologia mitosis/physiology epithelium corneal/growth &development corneal stroma/anatomy &histology imunohistoquÍmica/métodos apoptose corneal stroma/cytology corneal stroma/physiology microscopia eletrÔnica/métodos immunohistochemistry/methods microscopia confocal/métodos mitose/fisiologia microscopy fluorescence/methods epitÉlio da cÓrnea/anatomia &histologia epithelium corneal/anatomy &histology microscopy electron/methods

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