Estudo in vitro da compatibilidade do osso bovino misto quimicamente tratado

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Biomaterials have been developed and investigated aiming to help bone regeneration. Xenografts of bovine origin stand out due to their biocompatibility, low cost and availability. Some protocols have been tested to develop a chemically treated material containing the anorganic (hydroxiapatite) and the organic (collagen) components of bovine boned: the mixed bovine bone (MBB). In spite of the MBB has been shown as a promising material for dental and medical applications, due to its resistance and resilience, it must be certified that this material is atoxic and do not activate the immunological system of the organism. The purpose of this investigation was to evaluate the MBB cytocompatibility by the use of cytotoxicity tests (ISO 10993), morphological analysis and cell-material interaction studies. The cytotoxicity assays consisted of cell number determination, MTT reduction and Neutral Red uptake. In these tests, Balb/c 3T3 cell lines were treated with different concentrations of MBB extract (100mg/mL, 37oC, 24h). Statistically significant differences were observed among control group (no treatment) and concentrations higher than 50 mg/mL (p<0.01, ANOVA, Tukey?s test), similar to results observed for organic and inorganic bovine bone (Gen-Ox®). Morphological analysis (optical microscopy) confirmed these results. Cell-material interaction (scanning electron microscopy) was studied only after material dialysis and 72 h conditioning (Dulbecco Modified Eagle Medium, DMEM, plus 10% Fetal Calf Serum, FCS). Fibroblast proliferation and cell adhesion to the material were observed. We conclude that concentrations of MBB extract higher than 50mg/mL decreased significantly the cell number, mitochondrial and lisosomal function. Probably toxic residues from manufacturing procedures remain in the material, indicating that process optimization should be considered

ASSUNTO(S)

celulas - cultura biocompatibilidade materiais biomedicos

ACESSO AO ARTIGO

Documentos Relacionados