Estudo in silico de genes que codificam fatores de transcriÃÃo responsivos Ãseca, salinidade e congelamento nos genomas do eucalipto, cana e arroz

AUTOR(ES)

Nina da Mota Soares Cavalcanti

DATA DE PUBLICAÇÃO

2007

RESUMO

Abiotic stresses are the most responsable for causing changes in plant growth and development. The plants, in counterpart, make use of a variety of responses in order to maintain their metabolic and physiological processes. One of the main plants responses to environmental conditions alterations is the additional regulation of expression. Moreover, the biosynthesis of osmoprotectors, ionic transporters, chaperones, heat shock proteins, aquaporins, LEA proteins, among others, are of great importance. This work aimed to identify genes that encoding transcriptional factors (DREB, ERF, MYB, bHLH, bZIP, HSF, WRKY, NAC, ZincFinger and Homeodomain) in eucalyptus, sugarcane and rice through in silico approaches. The obtained results reveal the presence of transcription factors in all genome studied, revealing the importance of them in abiotic stresses responses. In general, the proteins belonging to the same family showed very similar characteristics (isoelÃtrico point and molecular mass), indicating the high conservation degree of them. The generated dendrograms reflected a relation much more adaptative than filogenitc among the organisms. Moreover, also the genic structure seems to be conserved in eucalyptus and sugarcane, as observed in comparative analysis between the ORFs of these cultures and of Arabidopsis and Oryza sativa genomic sequences. The found expression pattern reflect the involvement of these transcription factors in abiotic stress as much as in biotic stress, with great number of transcripts in tissues infected by Gluconacetobacter diazotroficans and Herbaspirilum rubrisubalbicans, stem, root and root-shoot transition zone. O padrÃo de expressÃo encontrado reflete o envolvimento destes fatores tanto no estresse biÃtico, quanto no estresse abiÃtico, com grande nÃmero de transcritos em tecidos infectados por bactÃrias, caule, raiz e regiÃo de transiÃÃo raiz-caule. On the whole, the results point for the conservation of main transcription control mechanisms involved in stress resistance/tolerance, like drought, salinity and cold, in eucalyptus, sugarcane and rice. However, additional researches, in vivo and in vitro, will give greater subsidies for the best explanation of protein functionality and the pathway in that these genes participate

ASSUNTO(S)

bioinformÃtica oryza sativa abiotic stress estresses abiÃticos saccharum officinarum oryza sativa eucalyptus genetica eucalyptus transcription factors saccharum officinarum fatores de transcriÃÃo bioinformatic

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