Estudo histoquimico da membrana sinovial da articulação temporomandibular

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

Morpho-functional studies are very important in understanding the pathogenesis of different diseases which affect the temporomandibular joint, however there are very few published studies about the histochemistry of the synovial lining cells. This work analyses the histochemistry of the synovial lining cells of the temporomadibular joint of adult female English Norfolk rabbits. We have developed techniques for dissection around the temporomandibular joint, including skin, subcutaneous tissue, and the masseter muscle. The removal of the zygomatic arch was an important step in sufficiently exposing the posterior region of the joint socket area for biopsy. The biopsies were chilled in n-hexane, frozen to . 70°C using liquid nitrogen, transferred by cryostat and sectioned in preparation for histochemical processing, using modified Gomori trichrome stain reaction, nicotinamide-dinucleotide-tetrazolium-reductase, dehydrogenated succinic, nonspecific sterase, acid phosphatase, Schiff periodic acid, alkaline phosphatase, alcian blue in pH 2.4 , and alcian-ponceau. Ultrastructural studies were also performed. The synovial lining cells showed activity in the different histochemical reactions listed above except for dehydrogenated succinic and alkaline phosphatase. The ultrastructural examination permitted clear differentiation between the A cells, similar to macrophages, and the B cells, similar to fibroblasts. The activity of the synovial lining cells to these different histochemical tests indicates the existence of complex biochemical reactions involved in the production and reabsorption of synovial fluid

ASSUNTO(S)

histoquimica articulação temporomandibular

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