Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco.

AUTOR(ES)

Soraya Farias de Andrade de Freitas

DATA DE PUBLICAÇÃO

2010

RESUMO

The aim of the present work was to detect the genetic polymorphism of the β-Lactoglobulin (β-Lg) in different groups of Holstein-Gir breed cattle. There was collected 165 animals blood samples from the experimental farms of the Instituto Agronômico de Pernambuco (Itambé e Arcoverde) and from a private farm located in Zona da Mata Sul in the state of Pernambuco. The genetic groups were 1/2, 3/4, 5/8 Holstein and 5/8 H 1st, 2nd, 3rd and 4th bi-crossbred generation. For the identification of genotypes, a located fragment was amplified in the areas of exons II and III of β-Lg gene using the PCR technique. The result of the amplification was submitted to the PCR-RFLP analysis to digest the fragment by HaeIII endonuclease and it produced fragments of 400, 220, 190, and 151pb molecular weight for all animals, equivalent to the heterozygous genotype (100% AB) of β-Lg. The Pearson s chi-square test (2)and considering the observed and expected genotypic frequencies of the genotypes β-Lg. Was verified that frequences differed significantly at the level tested (P<0,05), indicating that the samples studied was not in Hardy-Weinberg equilibrium.

ASSUNTO(S)

pernambuco (br) bovino girolando pcr-rflp proteína leite zootecnia milk protein pcr-rflp

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