Estudo de biodisponibilidade relativa de duas formulações de felodipina em plasma humano utilizando cromatografia liquida acoplada a espectrometria de massas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

A rapid, sensitive,robust and specificmethodwas developed for the determinationand quantitation of felodipine, in human blood plasma by liquid chromatography coupled with tandem mass spectrometryusing nimodipine as internalstandardoFelodipinewas extractedfrom 0.5mL human plasma by use of a liquid/liquidprocedureusing diethyIetherlhexane(80/20:v/v) as eluent. The method inc1uded a chromatographicrun of 5 minutes using a Cs analytical column (100 mm x 406mm iod.)and the calibrationcurve was linear over the range from 0.02 nglmL to 1OnglmL(~ >0.994). The between-run precision, determined as relative standard deviation ofreplicate qualitycontroIs,was 5.7%(0.06 nglrnL),7.1% (0.6nglrnL)and 6.8% (7.5 nglmL). The between-runaccuracy was :I: O, 2.1 and 3.1% for the above-mentioned concentrations,respectively

ASSUNTO(S)

biodisponibilidade espectrometria de massa cromatografia liquida

ACESSO AO ARTIGO

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