Estudo das proteínas da membrana eritrocitária de mamíferos de treze ordens da classe mammalia / Erythrocyte membrane proteins from selected mammals of thirteen orders

AUTOR(ES)

Elvira Maria Guerra Shinohara

DATA DE PUBLICAÇÃO

1996

RESUMO

Forty six different mammal species from thirteen regarding their erythrocyte membrane proteins, following data were observed: 1. No correlation was observed between mean corpuscular volume and spectrins concentration. 2. No correlation was observed between mean corpuscular volume and band 3 concentration. 3. It seems that there is inverse correlation between mean corpuscular volume and ankyrins concentration. Most of the studied animmals belonging to Artiodactyla, which exhibit low mean corpuscular volume, present higher ankyrin concentration. 4. A diffuse band in 4.5 region was observed among the Didelphis marsupialis, Arctocephalus tropicalis, Arctocephalus australis and Panthera leo when the ghosts were treated with 200 µM NEM, what was not found in the others species. This fact could be ascribed to hypothetical sequence of the Iysine, hystidine and cysteine which may be prone to NEM direct chemical action. 5. Although some qualitative and quantitative changes were observed among ali the studied species, ali of them seem to occur in the humans, disclosing that they are "house-keeping" proteins which participate as important pieces in the cytoskeleton structure and the membrane as well. 6. Ali the species presented two spectrins, with exception of Dasyprocta sp., which exhibited fours bands in the spectrins region. 7. The band 3 was the protein which showed the greatest variation, since 95 kDa in the Capra hircus up to 169 kDa in the Alouatta sp., as well as in the aspect, diffuse or well defined. 8. A great variability was observed in the 4.1/4.2 region, with apparent absence of one or another. Some Rodentia exhibited only one protein in the 4.1/4.2 region. 9. Among the Primates, the Hominoidea and the Cercopithecoidea presented an uniform pattern, but the New World monkeys (Cebus apella, Alouatta sp. and Ateies paniscus chamek) exhibited a striking molecular weight band 3 variation, although keeping the common diffuse pattern. 11. Ali the studied species did not present the evidence of protease action upon the membrane proteins, except the Didelphis marsupialis and Giraffa camelopardalis, which disclosed a proteolytic action upon the ankyrin.

ASSUNTO(S)

erythrocytes eritrócitos mammals mamíferos membrane proteins proteinas da membrana

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